Product & ReviewsAntibodies

Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot)

Product Details

Cat. No.
111-035-144
Type
Secondary Antibody
Clonality
Polyclonal
Host
Goat
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Jackson ImmunoResearch Laboratories Inc.

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Description

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species.

Biological Information

  • Clonality: Polyclonal
  • Host: Goat
  • Reactivity: Rabbit (Lapine)

Handling

  • Quantity: 1.5 ml
  • Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended us
  • Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
  • Specificity: 5

Applications

    Average Rating 5.0

    |1Scientists have reviewed this product

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    Write your own review

    Performs great with consistency

     

    Average Rating 5.0

    Application Area:

    Western blot

    This 2nd antibody works great, even it works when I used in only PBS-T for reducing background to get clean result using a dirty 1st antibody. It provided me consistent results.

    Review Date: 16 Sept 2016 | Jackson ImmunoResearch Laboratories Inc.

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