PINK1 Antibody
Product Details
- Cat. No.
- BC100-494
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![PINK1 Antibody [BC100-494] - Immunocytochemistry of PINK1 antibody (BC100-494 Lot G). HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a2c2aa81503a0f6dce53b2b1d1f9d7a90e98324d-400x302.jpg?w=1080&q=75&fit=clip&auto=format)
PINK1 Antibody [BC100-494] - Immunocytochemistry of PINK1 antibody (BC100-494 Lot G). HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP.
![PINK1 Antibody [BC100-494] - Immunocytochemistry of PINK1 antibody (BC100-494 Lot G). HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a2c2aa81503a0f6dce53b2b1d1f9d7a90e98324d-400x302.jpg?w=256&q=75&fit=clip&auto=format)
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Description
PINK1 Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Human, Mouse
- Antigen: PINK1
- Source: Immunogen affinity purified
Handling
- Quantity: 0.025 ml
- Storage: Store at 4C. Do not freeze.
- Buffer: PBS
Applications
- ELISA (ELISA)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Western Blotting (WB)
![PINK1 Antibody [BC100-494] - Immunocytochemistry of PINK1 antibody (BC100-494 Lot G). HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a2c2aa81503a0f6dce53b2b1d1f9d7a90e98324d-400x302.jpg?w=3840&q=75&fit=clip&auto=format)