PINK1 Antibody (8E10.1D6)
Product Details
- Cat. No.
- NBP2-36488
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody (8E10.1D6) [NBP2-36488] - HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% triton X-100 in PBS for 10 min. Cells were incubated with NBP2-36488 at 50 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Dylight-488 anti-mouse secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-rabbit DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP. Image objective 40x.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/df2f2929762676be131fab5a3213f039a8a6f015-400x303.jpg?w=1080&q=75&fit=clip&auto=format)
Immunocytochemistry/Immunofluorescence: PINK1 Antibody (8E10.1D6) [NBP2-36488] - HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% triton X-100 in PBS for 10 min. Cells were incubated with NBP2-36488 at 50 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Dylight-488 anti-mouse secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-rabbit DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP. Image objective 40x.
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody (8E10.1D6) [NBP2-36488] - HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% triton X-100 in PBS for 10 min. Cells were incubated with NBP2-36488 at 50 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Dylight-488 anti-mouse secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-rabbit DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP. Image objective 40x.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/df2f2929762676be131fab5a3213f039a8a6f015-400x303.jpg?w=256&q=75&fit=clip&auto=format)
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Description
PINK1 Antibody (8E10.1D6)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human
- Antigen: PINK1
- Isotype: IgG2b Kappa
- Source: Protein G purified
Handling
- Quantity: 0.025 mg
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS
Applications
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Western Blotting (WB)
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody (8E10.1D6) [NBP2-36488] - HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% triton X-100 in PBS for 10 min. Cells were incubated with NBP2-36488 at 50 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Dylight-488 anti-mouse secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-rabbit DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP. Image objective 40x.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/df2f2929762676be131fab5a3213f039a8a6f015-400x303.jpg?w=3840&q=75&fit=clip&auto=format)