protag-HiRes anti-GFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise ½, acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP,435, mVenus, Citrine.In protag-HiRes anti-GFP-X1, each fluorophore is coupled to exactly one single-domain antibody, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
- Clonality: Recombinant
- Host: Llama
- Reactivity: acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), paGFP, eYFP, eCFP, mTurquoise 1/2, mVenus, Cerulean, Citrine, Sirius, tSapphire, Superecliptic pHluorin, Superfold GFP
- Antigen: GFP
- Clone: 1H1
- Conjugate: Atto 565
- Isotype: single-domain antibody
- Quantity: 200 µl
- Storage: up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
- Immunocytochemistry (ICC)