protag-HiRes anti-GFP-X4 Atto 565, large

protag-HiRes anti-GFP is a blend of two camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise ½, acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP,435, mVenus, Citrine.In protag-HiRes anti-GFP-X4, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. The blend of two camelid single-domain antibodies therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our protag single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our protag-HiRes anti-GFP-X4 can thus improve the localization accuracy by 10-15 nm. Both features – superior brightness and precise fluorophore placement – render our protag-HiRes anti-GFP-X4 products excellent tools for all microscopy techniques.

• Clonality: Recombinant
• Host: Llama
• Reactivity: acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), paGFP, eYFP, eCFP, mTurquoise 1/2, mVenus, Cerulean, Citrine, Sirius, tSapphire, Superecliptic pHluorin, Superfold GFP
• Antigen: GFP
• Clone: 1H1/ 1B2
• Conjugate: Atto 565
• Isotype: single-domain antibody

• Quantity: 200 µl
• Storage: up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!

• Immunocytochemistry (ICC)