protag-HiRes anti-GFP-X4, large - select your fluorophore
Product Details
- Cat. No.
- 814xxL
- Type
- Primary Antibody
- Clonality
- Recombinant
- Host
- Llama
Stable HeLa cell line expressing YFP-fusion protein (A). Cells were stained with protag-HiRes anti-GFP-X1 Atto 488 (B, Cat. No. 81105) and protag-HiRes anti-GFP-X4 Atto 488 (C, Cat. No. 81405)(courtesy of NanoTag Biotechnologies GmbH).
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Description
protag-HiRes anti-GFP is a blend of two camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise ½, acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP,435, mVenus, Citrine.In protag-HiRes anti-GFP-X4, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. The blend of two camelid single-domain antibodies therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our protag single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our protag-HiRes anti-GFP-X4 can thus improve the localization accuracy by 10-15 nm. Both features – superior brightness and precise fluorophore placement – render our protag-HiRes anti-GFP-X4 products excellent tools for all microscopy techniques.
Biological Information
- Clonality: Recombinant
- Host: Llama
- Reactivity: acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), paGFP, eYFP, eCFP, mTurquoise 1/2, mVenus, Cerulean, Citrine, Sirius, Sirius, Superecliptic pHluorin, Superfold GFP
- Antigen: GFP
- Clone: 1H1/ 1B2
- Isotype: single-domain antibody
Handling
- Quantity: 200 µl
- Storage: up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Applications
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
