protag-HiRes anti-mScarlet-i-X2 Atto 542, large

protag-HiRes anti-mScarlet-i camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes mScarlet-i in its native conformation with high affinity and specificity. It can also recognize some mRFP-derived red fluorescent protein like mOrange2, dsRed, tdTomato, mRFP and mCherry in its native conformation. It does not cross-react with GFP or mTagBFP derivatives.In protag-HiRes anti-mScarlet-i-X2, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. protag-HiRes anti-mScarlet-i-X2 can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, the protag-HiRes anti-mScarlet-i-X2 can thus improve the localization accuracy by 10-15 nm. Both features – enhanced brightness and precise fluorophore placement – render the protag-HiRes anti-mScarlet-i-X2 products superior tools for all microscopy techniques.

• Clonality: Recombinant
• Host: Llama
• Reactivity: mRFP, dsRed1, dsRed2, mCherry, mOrange2, mScarlet-i, tdTomato
• Antigen: RFP
• Clone: 2B12
• Conjugate: Atto 542
• Isotype: single-domain antibody

• Quantity: 200 µl
• Storage: up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!

• Immunocytochemistry (ICC)