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protag-HiSec anti-chicken IgY is an isotype- and species-specific camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It is directed against chicken IgY and contains two site-specifically coupled fluorophores per protag molecule. One single-domain antibody targets two fluorophores to a primary chicken antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. There are no batch-to-batch variations, since the protag-HiSecs single-domain antibodies are recombinantly expressed.