Purified anti-γ-protocadherin (pan reactive)
Product Details
- Cat. No.
- 831701
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
Purified anti-γ-protocadherin (pan reactive); Clone: N159/5; Reactivity: Mouse; Apps: WB; Size: 100 μl
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Mouse
- Clone: N159/5
- Conjugate: PUREAF
- Isotype: Mouse IgG1
- Gene: 5098
Handling
- Quantity: 100 microl
- Storage: -20°C
- Specificity: 5
Applications
- Western Blotting (WB)
Tricky but specific.
Western blot
"Dirty antibody", do not use BSA for the blot. Use only 5% milk in TBST. Use TBS, Tween 0.2% instead of 0.1% to get cleaner membranes. dilutions: 1/1000 for primary, 1/5000 for secondary. Antibody is however specific. In this specific tissue, it shows 3 bands, the main band at 100kDa "full lenght", two light bands at 70kDa and 26kDa "N-ter cleaved" and C-ter cleaved respectively (theorically). These tree bands seem specific, cause my KO tissues show a significant increase in PCDHG expression (RNA-Seq data). Indeed, WB using this antibody confirmed their increased protein level. all 3 bands were higher in my KO tissues compared to WTs.
Review Date: 25 Jul 2018 | BioLegend
