R-Phycoerythrin-AffiniPure Goat Anti-Mouse IgG, Fc? Subclass 2c Specific (min X Hu,Bov,Rb Sr Prot)
Product Details
- Cat. No.
- 115-115-208
- Type
- Secondary Antibody
- Clonality
- Polyclonal
- Host
- Goat
Description
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2c but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Biological Information
- Clonality: Polyclonal
- Host: Goat
- Reactivity: Mouse
Handling
- Quantity: 0.5 ml
- Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day. Expiration date: six months from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
- Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
- Specificity: 5
Applications
Flow cytometry
The gamma2c-specific reagents are very useful for fusion proteins derived from the Invivogen pFUSE-mfc1 and -mfc2 expression vectors. Invivogen markets them as gamma2a Fc but they are not, they are gamma2c, meaning that they can be used in multicolor assays where the only other secondaries appropriate for another target are gamma2a isotypes.
Review Date: 30 Sept 2016 | Jackson ImmunoResearch Laboratories Inc.