RABBIT ANTI MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223)

Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody recognizes mitogen activated protein kinase (MAPK) 8 and 9, also known as c-Jun N-terminal kinases 1 and 2 or stress activated protein kinases 1 and 2, when phosphorylated on threonine 183 and tyrosine 185. This antibody also recognizes MAPK10, also known as c-Jun N-terminal kinase 3 or stress activated protein kinase 3, when phosphorylated on threonine 221 and tyrosine 223.As their name implies, MAPK8/9/10 are members of the serine/threonine MAP kinase family, which become activated by environmental stressors. In addition, activation through phosphorylation events has been reported upon stimulation of toll-like receptors and exposure to pro-inflammatory cytokines (Guma and Firestein 2012, Bogoyevitch and Kobe 2006).Two phosphorylation events are critical for the activation of the three MAPKs. The first is on threonine 183, or in the case of MAPK10 threonine 221, by dual specificity mitogen-activated kinase kinase 7 (MKK7) and the second is on tyrosine 185, or in the case of MAPK10 tyrosine 223, by MKK4 (Fleming 2000). The phosphorylation undertaken by MKK4 and MKK7 is counteracted by phosphatases of the MAP kinase phosphatase (MKP) family (Guma and Firestein 2012). The three MAPKs play a critical role in mediating cell signaling cascades by phosphorylating transcription factors, such as c-Jun and p53, thereby regulating cellular processes including proliferation and apoptosis (Guma and Firestein 2012).

• Clonality: Polyclonal
• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Polyclonal IgG

• Quantity: 50 µl
• Storage: -20ºC only
• Buffer: Phosphate buffered saline, 0.02% Sodium Azide, 50% Glycerol

• Western Blotting (WB)