RAT ANTI HUMAN CD3

Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).

• Clonality: Monoclonal
• Host: Rat
• Reactivity: Alpaca, Amazon Parrot, Amphibia, Avian, Bovine, Cat (Feline), Chicken, Cynomolgus Monkey, Dog (Canine), Duck, Harbour Porpoise, Horse (Equine), Human, Koala, Mammalian, Mouse, Pig (Swine), Rabbit (Lapine), Red-bellied Tamarin, Rhesus Monkey, Xenopus, Bullfrog, Great Horned Owl, Sea Lion, Spotted Hyena
• Isotype: IgG1

• Quantity: 0.2 mg
• Storage: +4ºC, -20ºC if preferred
• Buffer: Phosphate buffered saline, 0.09% Sodium Azide

• Flow Cytometry (FC)
• Immunofluorescence (IF)
• Immunohistochemistry (Frozen Sections) (IHC (Fr))
• Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
• Western Blotting (WB)