RAT ANTI MOUSE Ly-6A/E
Product Details
- Cat. No.
- MCA2782
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Rat
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Description
Rat anti Mouse Ly-6A/E antibody, clone D7 recognizes members of the lymphocyte activation protein-6 (Ly-6) multi-gene family. Specifically, it recognizes Ly-6A.2 and Ly-6E.1. Ly-6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly-6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells.Rat anti Mouse Ly-6A/E antibody, clone D7 has been reported to induce T cell activation. Removal of sodium azide is recommended prior to use in functional assays. We recommend the use of Slide-A-Lyser dialysis cassettes (EQU003) for this purpose.
Biological Information
- Clonality: Monoclonal
- Host: Rat
- Reactivity: Mouse
- Isotype: IgG2a
Handling
- Quantity: 0.25 mg
- Storage: +4ºC, -20ºC if preferred
- Buffer: Borate buffered saline, 0.09% Sodium Azide
Applications
- Flow Cytometry (FC)
- Immunohistochemistry (Frozen Sections) (IHC (Fr))
- Immunoprecipitation (IP)
- Western Blotting (WB)