Product & ReviewsAntibodies
SMAD6 Antibody
Product Details
- Cat. No.
- NB100-56440
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![Immunocytochemistry/Immunofluorescence: SMAD6 Antibody [NB100-56440] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SMAD6 (NB100-56440) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/31cea18e97ac80f3c817844a71541b080191539e-400x302.jpg?w=1080&q=75&fit=clip&auto=format)
Immunocytochemistry/Immunofluorescence: SMAD6 Antibody [NB100-56440] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SMAD6 (NB100-56440) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
![Immunocytochemistry/Immunofluorescence: SMAD6 Antibody [NB100-56440] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SMAD6 (NB100-56440) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/31cea18e97ac80f3c817844a71541b080191539e-400x302.jpg?w=256&q=75&fit=clip&auto=format)
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Description
SMAD6 Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Human, Mouse, Primate, Sheep (Ovine)
- Antigen: SMAD6
- Source: Protein G purified
Handling
- Quantity: 0.1 mg
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS
Applications
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blotting (WB)
![Immunocytochemistry/Immunofluorescence: SMAD6 Antibody [NB100-56440] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SMAD6 (NB100-56440) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/31cea18e97ac80f3c817844a71541b080191539e-400x302.jpg?w=3840&q=75&fit=clip&auto=format)