AnaSpec Introduces New SensoLyte uPA & tPA Assay Kits

Plasminogen activator (PA), classified immunological into urokinase-type PA (uPA) and tissue-type PA (tPA), converts the zymogen plasminogen to the active, broad-spectrum serine protease plasmin. Plasmin, in turn, mediates the pericellular proteolysis of extracellular matrix components and activates other proteases such as matrix metalloproteinases and collagenases that lead to the further degradation and remodeling of the extracellular matrix.

AnaSpec is pleased to introduce two plasminogen activator assay kits – the SensoLyte AFC Urokinase (uPA) Assay Kit and the SensoLyte AMC tPA Assay Kit. These AnaSpec SensoLyte assay kits, optimized for inhibitor screening, contain high-performance fluorogenic substrates which exhibit high reactivity and low background signal.

The AFC (7-amino-4-trifluoromethylcoumarin) labeled substrate in the AnaSpec SensoLyte AFC Urokinase (uPA) Assay Kit can be monitored at Ex/Em= 380/500 nm upon cleavage, with increase in fluorescence correlated to uPA activity. This substrate is not cleaved by tissue-type plasminogen activator (tPA). The AMC (7-amino-4-methylcoumarin) labeled substrate in the AnaSpec SensoLyte AMC tPA Assay Kit can be monitored at Ex/Em=354/442 nm. Upon protease cleavage, this substrate generates the AMC fluorophore emitting bright blue fluorescence. Increase in AMC fluorescence is proportional to tPA activity.

Figure: Inhibition of uPA activity measured with AnaSpec SensoLyte AFC uPA Assay Kit.