Applying Proteome Partitioning to Biomarker Discovery in Plasma or Serum: Immunoaffinity Enrichment Strategies

Separation of complex mixtures that have an enormous range of protein concentration, such as plasma or serum, is a significant challenge for proteomic analysis. Specific removal of highly abundant proteins (HAP) during sample preparation can improve the ability to detect low abundance proteins (LAP) and enhance biomarker discovery.

Immunoaffinity partitioning of HAP has proven to be one of the most effective approaches for this purpose. Compared to IgG, avian IgY (Immunoglobulin Yolk) antibodies have unique and advantageous features that improve specific protein removal from primate, rodent and other mammalian samples.

This talk will describe the efficiency and effectiveness of the ProteomeLab™ IgY Proteome Partitioning Systems to separate 12 highly abundant proteins from plasma using immunoaffinity columns in spin column and liquid chromatography (LC) formats, and will present several new applications.

The results demonstrated high specificity of protein separation, with removal of 95-99.5% of the abundant human proteins, and the ability to dig substantially deeper into the proteome.

Phenomenex distributes Beckman Coulter’s innovative IgY proteome enrichment chemistry to advance the discovery of protein biomarkers

Phenomenex Inc., a leading worldwide supplier of separation science consumables, announced today the completion of a distribution agreement for Beckman Coulter's market-leading ProteomeLab™ IgY enrichment products for proteomics and biomarker discovery. The agreement grants Phenomenex exclusive distribution of the ProteomeLab IgY product line in North America and countries in the European Union. "Protein partitioning is a critical part of any biomarker discovery and we view the ProteomeLab IgY products as the best available," commented Michael McGinley, Phenomenex Biochromatography Product Manager. "ProteomeLab IgY fits well with our portfolio of separation products for proteins and peptides providing complete solutions for proteomics workflows."