Innovative HP OnGuard siRNA Design ensures successful RNAi

HP OnGuard siRNA Design from QIAGEN is an upgrade to the HiPerformance siRNA Design Algorithm. It uses advances in the knowledge of the RNAi mechanism to reduce the risk of off-target effects and increase potency, ensuring successful RNAi.

HP OnGuard siRNA Design incorporates an innovative neural-network algorithm, proprietary homology analysis, and exciting, newly added features. siRNAs are available at QIAGEN’s GeneGlobe™ Web portal via the article webpage.

Design features include 3' UTR/seed region analysis, SNP avoidance, and interferon motif avoidance. siRNA design is reinforced using the results from QIAGEN’s siRNA validation project in which thousands of siRNAs have been tested for effectiveness by real-time RT-PCR. Data from Affymetrix® GeneChip® analysis is used to minimize the potential for off-target effects.

As well as siRNAs designed using HP OnGuard siRNA Design, matching RT-PCR assays, RNAi controls, and products for transfection and downstream analysis are available at GeneGlobe, covering the entire RNAi workflow.