Lonza to Highlight a New 3D Culture System Reforming Spheroid Culture and Analysis at AACR 2016

Lonza will demonstrate the RAFT™ 3D Culture System at AACR 2016 and highlight new applications and optimized protocols that allow researchers to overcome some of the challenges previously associated with working with 3D cultures.

RAFT™ 3D Cell Culture System uses a high-density collagen scaffold to closely mimic the extracellular matrix of native tissues. Cancer cells can be embedded in the collagen matrix, and many of them form tumor-like structures.

Delegates visiting the Lonza booth #1930 can discover:

• How the translucent properties of RAFT 3D Culture System enable visualization of tumor-like structures with subcellular resolution under a fluorescence microscope
• How modification of a standard 2D analysis method enables use of Lonza’s ViaLight™ Assays to assess cell viability in 3D cell culture.
• How the RAFT™ System permits different cancer cell types to show distinct morphologies, as well as characteristic tumor migration and invasion patterns. A recent study demonstrated how this can help researchers understand how to maximize the therapeutic potential of drugs in a more “in vivo”-like cell culture system.
• How to overcome the challenge of transfecting cells in 3D cell culture by using Lonza’s Nucleofector™ Transfection Technology to achieve high transfection efficiencies and good cell viability prior to transferring cells into 3D cell culture. This efficient approach creates spheroids that maintain transfected substrate for several days in culture

In addition, Lonza will present a poster in the tumor biology category titled “Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay” on Sunday, 17 April 2016, 1:00 pm to 5:00 pm, at the Convention Center, Halls G-J, Poster Section 28.

A further poster featuring the RAFT™ 3D Cell Culture System will be presented by Tarig Magdelin, Postdoctoral Research Associate at the Cancer Nanotechnology Group, University College London. “Engineering a Vascularized 3D "In Vitro" Model of Cancer Progression” will be presented on Tuesday, 19 April 2016, 1:00 pm to 5:00 pm at Poster Section 10.

More information about these new applications of RAFT™ 3D Cell Culture System for cancer research will be available at Lonza’s booth #1930 at AACR 2016.