Millipore Optimizes Protein Fractionation Using Ultrafiltration

Millipore Corporation today announced the development of a protocol for efficiently compartmentalizing protein solutions using ultrafiltration in a centrifugal mode.

Traditional fractionation techniques utilize size-exclusion chromatography, which is both laborious and time intensive. Comparatively, the new method confirms the advantage of speed and efficiency through a combination of Millipore’s Amicon® Ultra centrifugal devices and expertise.

Amicon Ultra centrifugal devices enable researchers to fractionate protein mixtures quickly and easily due to the product design. The bi-vertical housing limits membrane polarization, while the hydrophilic microstructure of low-binding Ultracel® ultrafiltration membrane enables efficient protein separation. This method expedites the fractionation process by minimizing process steps such as setting up columns, optimizing column flow rates, and collecting and concentrating various fractions.

Fractionation is a process for separating a mixture into its component parts to eliminate the complexity of the proteome. Common applications for this technique include biomarker analysis from serum, protein purification, 2D-PAGE and sample preparation for mass spectrometry analysis for those scientists in university, pharmaceutical and biomedical laboratories performing protein research.