Most Viewed Clinical Downloads of 2014

1. Five-Minute Vitamin D Analysis with Online Sample Cleanup
Sample preparation is generally the bottleneck in a laboratory’s analytical workflow. With online sample cleanup, sample turnaround time, robustness and data quality are significantly improved for the LC/MS/MS analysis of both vitamin D forms. This application note, from Agilent Technologies, describes a fast, sensitive, and selective LC/MS/MS method for quantitative analysis of 25-OH Vitamin D2 and D3 in serum and plasma.

2. A Practical Guide to High Resolution Melt Analysis Genotyping
HRM can be used to detect single-base sequence variations or discover unknown genetic mutations. This application note from Bio-Rad highlights the benefits and applications of HRM and discusses key considerations, such as instrumentation, sample preparation and experimental design.

3. Meeting Evolving Clinical Demands for Assay Performance: Advances in Acridinium Ester Technology on the ADVIA Centaur Systems
This white paper reviews the clinical and other benefits delivered by some of the latest acridinium ester (AE) dependent assays from Siemens Healthcare Diagnostics. The history of acridinium ester molecule evolution, reviewing specific details of AE properties, ongoing research findings, and modifications to the AE molecule that continue to drive critical advances in assay performance and diagnostic testing are also detailed.

4. Fast and Easy B Cell Isolation for More Sensitive CLL Assays
B-Cell Chronic Lymphocytic Leukemia (CLL) causes malignant cells to proliferate and accumulate in the bonemarrow and peripheral blood. Array-based comparative genomic hybridization (array-CGH) and array-based single-nucleotide polymorphism analysis (array-SNP) are increasingly common tools for profiling genetic abnormalities in CLL and other haematological malignancies. This white paper discusses the advantages of these assays over traditional techniques.

5. Determination of Sulphur in Nasal Spray and Na₂S • 9 H₂O
Sulphur has a positive influence on chronic diseases; it is anti-inflammatory and stimulates wound healing. In order to apply the agent on the affected areas, the use of spray bottles has been established. For ensuring an effective and safe dosage, the quality of raw materials, as well as the final products, have to be controlled regularly. In this application note from Analytik Jena, nasal sprays from different batches and the raw sulphuric salt (Na₂S • 9 H₂O) were examined for their sulphur content.

6. Solutions for Efficient Sample Management and Biobanking
Modern biobanks usually contain a vast number of samples, as well as a wealth of sample-related information. This application note provides an overview on the Cryo.s™ product line, including cryogenic storage tubes and racks.

7. Improved Extraction and LC/MS/MS Analysis of Barbiturates from Urine Samples Using Strata™-X-Drug N SPE and Kinetex® Core-Shell HPLC/UHPLC Columns
The Strata™-X-Drug N SPE sorbent from Phenomenex is shown to provide both time and solvent savings, which can be multiplied when screening several samples at once, making it faster and more profitable for forensic labs to screen barbiturate samples. By pairing this improved extraction with a sensitive LC/MS/MS method on a Kinetex 2.6 μm C18 core-shell HPLC column, labs can resolve all five barbiturates screened, including pentobarbital and amobarbital, which has proved difficult using older HPLC methods.

8. MammaPrint and BluePrint in Early Breast Cancer: Clinical Implications of Prognostic Stratification and Molecular Subtyping
This poster describes a study to compare tumor subtyping methods of clinical immunohistochemistry (IHC)/fluorescent in situ (FISH) hybridization, and MammaPrint and BluePrint, and to determine which method of subtyping most accurately predicts long term outcome.