New Application of Dyversity: A Rapid Method of Imaging Deep Purple Stained Protein Gels

Syngene, a world-leading manufacturer of image analysis solutions, is pleased to announce Dyversity, its new multi-functional imager, can now detect protein stained with Deep Purple™ in seconds. This will benefit scientists wanting a quick, affordable method of automating gel-based proteomics research.

Using a Dyversity system fitted with a Cy dye lighting module, dual wave length transilluminator, UV, long pass and Cy3 dye emission filters, Syngene’s technical team imaged 1D acrylamide gels containing 1000-0.1ng of molecular weight standard PeppermintStickä from Invitrogen. The gels were imaged with three different settings: Cy3 excitation with a Cy3 emission filter; Cy3 excitation with a long pass emission filter and long wave UV excitation (365nm) with a UV emission filter.

The technical team found all three imaging settings of Dyversity could detect as little as 20ng of Deep Purple stained protein. However, using long wave UV excitation with the UV emission filter, Dyversity produced an image in less than 2 seconds, 10 times faster than either of the other imaging conditions used.

Dyversity can image Deep Purple stained proteins so rapidly because its high performance 16 bit, 6.3 mega pixel camera captures a wide range of signal emission wavelengths from the whole gel simultaneously. Additionally, since Dyversity can be fitted with a variety of lighting and emission filter options it is easy to achieve the right conditions to accurately image many different protein and DNA stains.

Laura Sullivan, Syngene’s Divisional Manager stated: “We are excited that Dyversity can rapidly detect small amounts of Deep Purple stained proteins. Dyversity offers such a wide range of imaging conditions that unlike using a laser based scanner, scientists can take advantage of exciting at the optimum light excitation peak and this can be either UV or visible. This means Dyversity is versatile enough to quickly image both fluorescent and visible dyes with ease, making it an excellent, cost-effective alternative for proteomics gel applications.”