New Hypersil GOLD HILIC Columns for Retention of Highly Polar Compounds

Hydrophilic interaction liquid chromatography (HILIC) is becoming an increasingly popular technique that offers complementary selectivity to reversed-phase. With the ability to retain highly polar and hydrophilic compounds, this new HILIC range of columns has been developed to aid the analysis of compounds that are traditionally difficult to retain using conventional C18 columns. Available with 1.9, 3 and 5 µm particle sizes, Hypersil GOLD HILIC columns are ideal for applications within drug discovery, food testing and environmental analysis.

HILIC works by incorporating water in the highly organic mobile phase to form, an adsorbed water-rich layer on the polar stationary phase surface into which analyte molecules partition. Retention is governed by dipole-dipole interactions and hydrogen bonding mechanisms. Furthermore, the highly organic mobile phase contains low salt levels, making Hypersil GOLD HILIC columns ideal for use with electrospray mass spectroscopy.

With a wealth of in-house chromatography expertise within its new dedicated chromatography research and manufacturing facility in Runcorn, UK, the new Hypersil GOLD HILIC range and the established Thermo Scientific Hypercarb columns, Thermo Fisher Scientific offers a complete solution for difficult-to-analyze compounds. Tony Edge, technical manager, Thermo Fisher Scientific, commented, “We have an expanding scientific technical team in place to assist users globally, particularly with problem analyses. Introducing the HILIC columns furthers our already extensive HPLC and UHPLC product offering, enabling us to build stronger customer relationships by advising on the use of our even broader range of analytical options."