New Kit to Facilitate RNA Analysis in Cell-Free Fluids

The ExoMir™ kit from Bioo Scientific allows the fractionation of and RNA extraction from exosomes and other microparticles in cell-free fluids. The release of these microparticles may increase in number and type during pathological conditions, especially malignancies. These tumor exosomes contain nucleic acid sequences of a cancer's genetic material.

Traditional methods to recover exosomes require ultracentrifugation of samples at high centrifugal force (~100,000 x g or more) for at least several hours to pellet the particles. The ExoMir Kit uses an alternative approach in which samples (~ 10 mL – 30 mL) are passed over syringe filters to capture exosomes and larger membrane-bound particles. The filters are then flushed with BiooPure™-MP , an RNA extraction reagent, to lyse the captured particles and release their contents. Recovery of the low mass amounts of RNA from the lysates is maximized by using the inert co-precipitant included in the kit. Cell-free fluids that can be processed with the ExoMir Kit include blood serum, cerebrospinal fluid, and eukaryotic cell culture media.

According to Dr. Marianna Goldrick, Senior Scientist at Bioo Scientific, “The ExoMir kit significantly reduces the labor and costs required for microRNA profiling on particles recovered from cell-free fluids. When used along with Bioo Scientific’s MiraMas™ kit for qPCR detection of small RNAs, researchers can rapidly and inexpensively carry our small RNA profiling studies using a variety of cell-free fluids.”