New qPCR master mix portfolio to maximize real-time PCR experiment specificity and reproducibility

Thermo Fisher Scientific continues to innovate its SYBR Green real-time PCR (qPCR) master mix portfolio with the launch of the Applied Biosystems™ PowerTrack™ SYBR™ Green PCR Master Mix. The PowerTrack SYBR Green Master Mix is a pre-formulated, universal, 2X concentration Master Mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplify targets for accurate gene expression analysis. The master mix contains a two-color tracking dye system designed to help researchers keep track of where they have pipetted. The master mix contains an inert blue dye and a separate, optional yellow sample buffer. The yellow sample buffer is added separately to indicate that sample has been added to the reaction, based on a visual color change of the reaction mix from blue to green.

PowerTrack SYBR Green Master Mix uses an antibody-mediated hot-start mechanism to provide tight control over Taq enzyme activation and help prevent early activity of the polymerase at low temperatures that can lead to nonspecific amplification. For researchers using qPCR for gene expression analysis, specific amplification of target genes is important. The inclusion of UNG and dUTP enzymes also prevents previously amplified PCR products from contaminating subsequent qPCR reactions.

PowerTrack™ SYBR^® Green Master Mix provides robust results ranging from 100 ng to 0.1 pg of complementary DNA (cDNA) per reaction. It can be used in either standard or fast cycling mode and is compatible with all qPCR instruments. Dynamic range is a property of both the assay and template concentration in the sample, as well as in the master mix, thus individual results may vary.

Features/Benefits:

• Built-in two-color tracking dye system for precise pipetting in reaction set up

• Broad primer Tm and primer concentration compatibility allows flexibility in qPCR reaction set up with minimal optimization

• Superior specificity and tight reproducibility in Ct values over a broad dynamic range improve data quality

• Compatible with SuperScript IV VILO Master Mix reverse transcription for fast, reproducible results

• Compatible with standard or fast cycling for results in less than 50 minutes

• Formulated with UNG/dUTP to prevent contamination of carryover PCR products

• Broad instrument compatibility

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