Novus Biologicals Announces New Antibody for Stem Cell Research

Novus Biologicals announces a new rabbit polyclonal anti-Lin28 antibody for stem cell research (catalog # NBP1-49537). This antibody is useful for immunofluorescence, immunohistochemistry and Western blot.

Novus' new Lin28 antibody is useful for immunofluorescence, immunohistochemistry and Western blot. By Western blot, Lin28 antibodies recognize a band at approximately 29kDa. This new Lin28 antibody was validated by Western blot on NTERA-2 cell lysate and 293T cells overexpressing LIN28. This Lin28 antibody cross-reacts with human and mouse. The immunogen to which this antibody was made is a partial recombinant human Lin28 protein [Swiss-Prot# Q9H9Z2] expressed in E. coli.

Lin28 is marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem cells from human fibroblasts. Lin28 acts as a 'translational enhancer,' driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis.

As an embryonic stem cell marker, Lin28 is closely related to Sox2, OCT4 and Nanog.