Online Core-Shell HPLC Protein Separation and Top-Down Proteomics Using High Resolution Mass Spectrometry

Top-down proteomics applications have been developed using a new, revolutionary core-shell based HPLC column (Protea Amplus®) for online separation of proteins. This poster presents the separation of several intact proteins by HPLC-MS as well as characterization of glycosylated or non glycosylated protein proteoforms.