Sick of wasting time, effort and money on secondary reagents?

The Lightning-Link™ kit allows antibody conjugates to be set up in seconds, simply by adding a solution of the protein to be labeled to a proprietary lyophilised mixture containing Alkaline Phosphatase (AP). By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, Lightning-Link™ technology can be used to label small quantities of protein with 100% recovery and without excessive dilution of the conjugate!

Upon dissolution of Lightning-Link™ mixture with a solution of the antibody (or other biomolecule to be labeled) proprietary chemicals in the mixture become activated. This results in the directional coupling of the antibody to the enzyme label in a gentle and controlled process at near-neutral pH. The hands-on time for the entire procedure is typically 20-30 seconds. Lightning-Link™ makes it possible to label primary antibodies and other proteins with ease, and eliminates the need for secondary reagents in immunoassay procedures such as Western blotting, ELISA and immunocytochemistry.

Alkaline phosphatase (AP) is a hydrolyase enzyme that is frequently conjugated to antibodies for use in immunoassays. AP has a lower catalytic rate than HRP, but has greater stability and maintains linear reaction kinetics for much longer. PNPP is a popular colorimetric substrate for AP; the product of the reaction, p-nitrophenol, can be read at 405nM. Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate; MUP) may also be employed.

Please note as well as AP, we currently have over 40 available labels including enzymes, fluorescent proteins and dyes that span a variety of applications (WB, IH, FACS, ELISA, and FRET), colours, and emission ranges