The Latest Cutting Edge Cancer Methods Available for Download
The SelectScience article library contains downloads on the very latest technologies being used in cancer research and diagnostics
10 Feb 2016
The SelectScience application article library is updated daily with new case studies, white papers, scientific posters and application notes
Scientific advances in the field of cancer diagnostics are rapidly progressing. This article highlights some of the most interesting new articles available to download from the SelectScience library including topics such as next generations sequencing and liquid biopsies.
1. Somatic Variant Detection from Liquid Biopsy Samples using Targeted NGS
This application note describes a robust method for detecting somatic mutations from liquid biopsy samples by combining circulating tumor cell (CTC) enrichment with a high sensitivity targeted next-generation sequencing (NGS) panel. The combined workflow has broad implications for how cancer can be monitored non-invasively using a routine blood draw without the need for tissue biopsies.
2. Fast and Reliable Real Time PCR Quantification of Leukemia Associated Genes
The aim of this study was to automate the quantification of gene expression in leukemia cells focusing on 140 genes that affect telomere length, apoptosis control, NF-kB pathway and protein degradation. The study used samples for patients with acute myelogenous leukemia (AML) and acute lymphoblastic leukemia (ALL) at different stages of progression and treatment.
3. Correlation of Mutations Detected in Liquid and Tissue Biopsies Using the Maxwell® RSC
This application note describes a method for purifying nucleic acids using the Maxwell® RSC instrument, based on novel surface and binding chemistries. Circulating cell-free DNA (ccfDNA) in plasma can be used to detect biomarkers that show great promise for diagnosis and monitoring of cancer, giving rise to the possibility of liquid biopsies that obviate the need for invasive tissue collection.
4. Circulating Cell-Free DNA and Circulating Tumor Cell Detection on the MassARRAY® System
Circulating cell-free DNA (ccfDNA), isolated from the plasma portion of a blood draw, can originate from apoptotic and necrotic tumor cells, whereas circulating tumor cells (CTCs) are living cells that have shed from the primary tumor and circulate in the bloodstream. This application note answers some frequently asked questions regarding ccfDNA and CTCs and demonstrates the application of the MassARRAY® system in their study through the use of case studies.
In this application note, the SNAP i.d.® 2.0 Protein Detection System for multi-slide screening with immunohistochemistry (IHC) to perform screening of an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.
6. A Solution for The High Content Screening Analysis of Neutrophil Chemotaxis Assays
In this document BellBrook Labs describe studying neutrophil migration using an iuvo Chemotaxis Assay plate with TTP LabTech’s Acumen eX3 high content imager, and inverted Nikon Eclipse TE2000U microscope is also used for image acquisition.