The Lightning-Link™ Story – the World’s Easiest to Use Conjugation Kits

Innova Biosciences through the Lightning-Link™ range fosters pioneering research by empowering scientist to develop novel antibody research tools within their own laboratories. The Lightning-Link™ range has 43 individual signal labels, including enzymes, fluorescent proteins and dyes that span a variety of applications (WB, IH, FACS, ELISA, and FRET), colours, and emission ranges.

As we move from the post-genomic era in to the proteomics arena, scientists are shifting away from studying single proteins to multi-component research for instance RNA splicing and transcription factories. However, progress within the Life Science community has been protracted by the lack of commercially relevant antibodies which are directly labelled. Conventional indirect methods (secondary antibody detection) have failed to evolve to meet this emerging demand and often, such techniques are difficult to optimise when analysing multi-component complexes in experiments such as Western-Blotting, Immunofluorescence, Immunohistochemistry and FACS.

The solution to these problems is via direct labelling of the primary antibody using conjugation. However, in the past conjugation specialists were required for a successful outcome, development times varied between 1-3 days and multi-step approaches were required for instance desalting, and purification columns (potential loss of precious antibody resource). Furthermore, normal conjugation technologies require bulk volumes of mg; researchers may only require µg of labelled antibody.

Recognising this emerging demand and through innovation, Innova Biosciences (UK, Cambridge) developed the lightning-link™ kit which enables the end-user to generate primary labelled antibody conjugates with a hands-on time of 30 seconds in a one step format, greatly reducing preparation times. Additional benefits include scalability (µg to mg) and 100% antibody recovery. In essence, Innova Biosciences has developed the world’s easiest to use conjugation kit to meet the emerging needs of the research community.