Thermo Fisher Scientific launches next-generation Invitrogen TaqMan Cells-to-CT Express Kit
25 Jan 2024
To help labs pursue more sustainable practices, Thermo Fisher Scientific has announced a next-generation Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit, offering simplified, scalable workflows for gene expression analysis that produce less plastic waste. With improved chemistry, the Cells-to-CT Express Kit helps facilitate the preparation of cell lysates for reverse transcription (RT) real-time PCR (qPCR) in just five minutes.
RT-qPCR is commonly performed to support gene expression analysis, which compares the expression of genes between two or more samples and can help researchers determine gene expression targets for additional study. In turn, gene expression analysis can enable and guide more efficient drug development research.
With the Cells-to-CT Express Kit, researchers can measure relative gene expression while skipping the traditional RNA purification step when performing RT-qPCR. Instead, with only five minutes of prep time required, they can go directly from cultured cells to RT-qPCR. This not only promotes a faster workflow overall, with results delivered from an entire plate of cells in 70 minutes, but also reduces the plastic waste generated from the process.
With the Cells-to-CT Express Kit, the amount of waste generated is approximately 8.1 grams of plastic. In contrast, with a traditional RNA purification kit, the waste generated is approximately 106.9 grams. This is equivalent to a 92.4% reduction in plastic waste when using the Cells-to-CT Express Kit.