Thermo Fisher Scientific Publishes New Poster Demonstrating the Reduction of Matrix Effects in LC-MS/MS Analysis for Drug Discovery and Development

Thermo Fisher Scientific Inc., the world leader in serving science, today announced the publication of a new technical poster titled “Ion Suppression and Matrix Effects.” The poster shows how Thermo Scientific TurboFlow™ technology in combination with the TSQ Quantum™ family of triple quadrupole mass spectrometers can reduce matrix effects in liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis. The new technical poster and a companion CD, titled "LC-MS/MS Solutions for Drug Discovery and Development," are available free-of-charge from the article webpage.

The poster discusses ion suppression relative to various sample clean-up procedures, and shows data from the same experiment performed on samples prepared by Solid Phase Extraction (SPE) and Protein Precipitation (PPT). Samples run on the Thermo Scientific Aria™ TLX-1 system with TurboFlow technology and analyzed on the TSQ Quantum Ultra™ mass spectrometer showed the least ion suppression and required the fewest sample preparation steps. TurboFlow technology can assist pharmaceutical researchers in complying with U.S. FDA/CDER Bioanalytical Method Validation Guidance that states: "In the case of LC-MS-MS-based procedures, appropriate steps should be taken to ensure the lack of matrix effects throughout the application of the method, especially if the nature of the matrix changes from the matrix used during method validation." When configured to the Thermo Scientific TSQ Quantum, TurboFlow technology delivers fast and efficient quantitative results with the lowest ion suppression and chemical noise, resulting in highly sensitive, robust bioanalytical methods and reduced analysis failures.

This unique solution allows scientists to reduce matrix effects by minimizing sample preparation for LC-MS/MS analysis in drug discovery, development and clinical research applications. TurboFlow technology can be used as an online sample preparation technique or for direct injection.

The companion CD includes several animations, including demonstrations of how FAIMS (High-Field Asymmetric Ion Mobility Mass Spectrometry) lowers chemical noise and enhances sensitivity during quantitation experiments, how TurboFlow technology allows direct injection of biological samples into an MS/MS system and how a heated electrospray ionization (H-ESI) probe enhances LC-MS/MS assay sensitivity up to eight times.