UHPLC+ Solutions for Inverse Gradient: Uniform Response with UltiMate 3000 x2 Dual System and Charged Aerosol Detection

Dionex Corporation is pleased to announce the release of the UltiMate® 3000 x2 Dual UHPLC+
Solution for Inverse Gradient to achieve a uniform response in liquid chromatography with charged aerosol detection (CAD®).

The application of the inverse gradients allows quantification of compounds even if there is no standard available for them and before the elucidation of their chemical structure. In this solution, the Dionex Corona® ultra™ detector or the newly introduced Corona ultra RS™ detector provides nearly universal response for nonvolatile and some semi volatile analytes and is also fully compatible with the high analysis speed of the UHPLC+ system.

This turnkey solution provides isocratic conditions even when running gradient chromatography. The unique UltiMate 3000 x2 system applies the inverse of the analytical gradient after the column, but before the Corona detector. The effect of this is to provide consistent response of all non-volatile analytes (and some semi-volatile analytes) independent of the chemical structure of the analytes and of the analytical gradient used. With a consistent response, unknown compounds can be quantitated either through their relative area, or by using a generic response curve of a known analyte.

“There are multiple applications for this technique across many markets,” said Frank Steiner, LC Solutions Manager for Dionex. “It is already used for metabolite research and stability testing in the pharmaceutical market. In addition, such a technique can be used for providing quantitative information on antibiotics in surface water or lipid profiles in edible oils. We see it being adopted by multiple laboratories for multiple applications.”

The new Corona ultra RS detector includes features that enhance the usability and extend application use, such as an on-board switching valve that allows the detector to be used independently of other detectors, without the need for system reconfiguration. The same valve can be used to divert unwanted components from samples. An optional variable ratio flow splitter is available to make it easier to use the CAD in parallel with MS without compromising resolution. The Corona ultra RS detector is fully compatible with HPLC and UHPLC methods and provides a consistent response independent of the molecule’s chemical structure for nonvolatile and some semi volatile analytes with or without a chromophore.

The UHPLC+ Solution for Inverse Gradient kit includes all necessary fluidic parts for inverse gradient including the unique Dionex Viper™ and nanoViper™ fingertight connectors for zero dead volume connections. The enterprise ready Chromeleon® Chromatography Data System (CDS) software version 7.1 with its unique eWorkflows tool supports the perfect match of the two combined gradients independent of the separation column volume resulting in a solution with the highest operational simplicity possible.

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