Waters Introduces Two New Column Chemistries for Protein Separations

Waters Corporation announced today the introduction of new 300Å, reversed-phase columns for protein separations on traditional high performance liquid chromatographs or Waters ACQUITY UltraPerformance LC^® (UPLC^®) systems. The XBridge™ BEH300 C4, 3.5 µm and ACQUITY UPLC BEH300 C₄, 1.7 µm columns deliver substantially greater protein resolving power and performance as compared to traditional 100-percent silica-based particle materials. These columns are now available for immediate delivery on a worldwide basis.

The first in a series of Protein Separation Technology columns, these columns are based on its second-generation, patented, highly-stable Ethyl Bridged Hybrid (BEH) particles that have set new standards for LC and UPLC separations. The results of accelerated stability tests confirm that the BEH300 C₄ columns last longer and are more stable under low and high pH conditions than many competitive column offerings when tested under identical conditions. Other features of the BEH300 C₄ columns include:

• Separates proteins of various sizes, hydrophobicities, and isoelectric points yielding good peak shape
• Surface chemistry that minimizes undesirable secondary interactions
• Enhanced pH- and temperature-tolerant characteristics
• Rigorously quality-control-tested with proteins to help ensure consistent batch-to-batch performance
• Maintains protein resolution with LC/MS-compatible eluents

For additional information on ACQUITY UPLC BEH300 C₄, 1.7 µm and XBridge BEH300 C₄, 3.5 µm Columns, visit the company article page.