In the rapidly advancing fields of pharmaceuticals, biotechnology, and life sciences, the integrity and accuracy of analytical processes are vital. When analyzing substances like peptides and nucleic acids, even minimal interactions between the sample and analytical hardware can lead to non reproducible results and in some cases even degradation. The analysis of metal-coordinating compounds, phosphorylated lipids and peptides and biomolecules like oligonucleotides often result in reduced recovery, carryover, and poor peak shape due to interaction with metal ions in stainless steel column hardware. This highlights the importance of utilizing precision-engineered analytical hardware to generate high-quality and reliable results and maintain sample integrity.

Harness bioinert hardware

Bioinert hardware components are specifically designed to minimize interactions with sensitive (biological) samples, ensuring that the analysis of a sample remains accurate and reliable. Raising awareness about the necessity of these specialized tools is crucial for advancing research and maintaining the highest standards.

In this content hub, explore a range of free resources on bioinert hardware and discover how YMC Accura columns' coated bodies and frits prevent analyte interaction with metal ions. Additionally, learn how to achieve enhanced sensitivity and recovery with stable, sharp peak shapes from the initial injection, saving both time and samples.

The analysis of biomolecules, especially those containing phosphate groups, is common across numerous scientific disciplines, including pharmaceutical sciences and genomics. These biomolecules include (phospho-)peptides, proteins, antibodies, nucleotides, and oligonucleotides. Interactions between biomolecules and stainless steel hardware can significantly impact results. Fortunately, specialized equipment is available to prevent undesired interactions, preserve sample integrity, and ensure reliable analytical results.

YMC Accura hardware is engineered to prevent analyte interaction with metal ions. This technology delivers enhanced sensitivity and sample recovery, providing stable and sharp peak shapes right from the initial injection. With no requirement for pre-conditioning, this technology saves valuable time and preserves precious samples.

Explore the resources below to discover how to significantly enhance peptide analysis, prevent unwanted ionic interactions, and even analyze virus proteins to ensure drug safety.

Oligonucleotides have become more important in forensics, genetic testing, and research, and to date, a variety of oligonucleotide-based approaches have been developed for different pharmaceutical applications. This has required more robust and highly sensitive analytical methods. Ion pair reversed phase liquid chromatography remains the gold standard. However, conventionally used stainless steel for tubing and column hardware poses a challenge for the analysis of oligonucleotides.

Stainless steel is durable and works with most solvents, but solvents like methanol or acetonitrile can cause corrosion. This corrosion can create a positively charged surface, leading to metal leaching and unwanted ionic interactions with analytes. Oligonucleotides, with their electron-rich backbone, can be irreversibly adsorbed. The resources below explain how these issues affect recovery and peak shape and offer solutions to address them. Plus, learn why anion exchange chromatography (AEX) is ideal for oligonucleotide analysis and find out how to overcome common issues.

The separation of certain small molecules can be significantly influenced by the choice of stainless steel column hardware. For example, metal-coordinating compounds, such as chelating agents, can interact with the metallic surface of the column, potentially compromising the accuracy of the analysis.

In the resources below, we examine a study that used a bioinert YMC AccuraTriart Diol-HILIC column for non-targeted screening of polar metabolites in human plasma. The bioinert coating on the stainless steel hardware is crucial for achieving maximum sensitivity and resolution, particularly for separating endogenous isomers. Plus, we highlight how bioinert column hardware impacts small molecule analysis of chelating agents and phospholipids.

When YMC stationary phases are paired with bioinert YMC Accura hardware, users benefit from enhanced efficiency and reproducibility in their analyses. This combination improves data reliability and streamlines workflows, making it an asset for any lab focused on high-precision analytical processes.

Bioinert RP/HILIC (U)HPLC Columns: YMC Accura Triart and YMC-Triart metal-free

RP and HILIC bioinert YMC Accura Triart columns are available for the analysis of critical substances including selected proteins, (oligo)nucleotides and metal-coordinating compounds.

Versatile, pH Stable (U)HPLC Columns: YMC-Triart Portfolio

Robust and reproducible (U)HPLC columns with a wide range of stability towards pH (1–12), temperature and 100% aqueous eluents.

Robust bioinert columns for a new level of recovery: YMC Accura BioPro IEX columns

Bioinert coated YMC Accura BioPro IEX columns provide excellent peak shapes for sensitive compounds, ensuring high recovery from the very first injection.

High Resolution Ion Exchanger: BioPro IEX

BioPro IEX, YMC's strong ion exchange materials are based on a hydrophilic polymer which provides low non-specific adsorption and high recoveries. Porous and non-porous polymer beads are available for high loadability and throughput.