1-Drop PCR Master Mix
The easiest and most convenient way to run PCR: squeeze one drop then run PCR.
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Fast and reliable. Really makes a difference!
Genotyping and cloning
1-Drop PCR Master Mix has really changed our lives (especially the life of our lab tech). We have multiple lines of mice that we routinely genotype. It's dozens of samples per week. Not only this kit makes it easier and faster to prepare the PCR reaction (just 1 drop - no need to mix multiple reagents), but it also provides very robust bands with fewer cycles. For example, genotyping for Cre expression requires only 20-25 cycles, unlike the 30-35 cycles we used with the "usual" Taq polymerase. Moreover, I was also very impressed that I was able to get a very good band from the first attempt when I tried to amplify a much longer fragment (1.5 kb) for cloning (note that this PCR mix is based on the Taq polymerase, so the fragment can be subcloned into the TA vector). One more thing - it looks like this kit is not too sensitive to the differences in the Tm (compared to the kit with the Phusion polymerase, for example) - pretty much all the gradients we did with this One-drop-PCR produced a nice expected band - less room for errors and less time for troubleshooting and trying to find the optimal conditions. Overall, I highly recommend this master mix for both genotyping and cloning.
Review Date: 16 Jun 2021 | 101bio
The easiest and most convenient way to run PCR: squeeze then run.
User can squeeze one drop of PCR Master mix into PCR tube/plate, add template and primers, and then can run PCR reaction. This PCR Master Mix contains Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. This unique Mix recipe makes the system very reliable, and tolerant to most of the PCR condition (concentration of the components).
The Taq Polymerase in the PCR Master Mix is Hot Start polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. So no need to prepare the reactions on ice. The Polymerase is activated by a 10 minutes, 95°C incubation.
This PCR Master Mix is very stable, and can be stored in 4oC for up to 6 months. No need to wait the thawing.
The amplification range of the Taq is ~ 6 kb.
The PCR Master Mix contains dye, and can directly run electrophoresis after PCR reaction.
