2-Mercaptoethanol
For molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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Would purchase from no other brand!
Electophoresis
This was one of the best mercaptoethanols we had used! It provided excellent results every time! We stopped using other brands and only use Millipore Sigma's.
Review Date: 16 Jul 2021 | Merck
Application
Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
- as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
- to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
- to trypsinize mouse embryonic stem cells
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
