ACQUITY UPLC® BEH HILIC Columns
ACQUITY UPLC® BEH HILIC columns contain the rugged 1.7 μm unbonded BEH particles and are designed to retain and separate very polar basic compounds.These unique columns are optimized and tested to produce efficient and reproducible separations under UPLC® HILIC conditions. ACQUITY UPLC BEH HILIC columns overcome a major weakness of HILIC stationary phases: chemical stability. The unbonded silica stationary phases often used in…
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Great product.
Various HILIC applications for LC-MS of polar analytes
Very efficient separations, we have used the columns for more than 12 years. We practically base more than 12 protocols on sugars, aminoacids, amines, nucleotides analysis. Reproducible and long life.
Review Date: 30 Oct 2020 | Waters
The column is good for small molecule separation.
Separation of compounds so that they can be detected later in detector
This column is easy to maintain. The perfomance of this column is suitable for the separation of small polar molecules. We used this column to do metabolomic analysis.
Review Date: 26 Mar 2019 | Waters
LC-MS/MS endocrinology
It is not very easy to work with HILIC columns, but it does works. If I had to choose now, maybe I would prefer to do reverse phase chromatography for this application. The HILIC amides are the more robust columns.
Review Date: 27 Jan 2014 | Waters
QC Analysis
Columns have low use value, they don't tend to last long. However, a short run time is a plus and it saves time on long analyses.
Review Date: 22 Mar 2012 | Waters
I like to use this column for my work. It works well and the only obstacle is having to prepare salt solution although this is not too much work.
Review Date: 13 Apr 2011 | Waters
ACQUITY UPLC® BEH HILIC columns contain the rugged 1.7 μm unbonded BEH particles and are designed to retain and separate very polar basic compounds.
These unique columns are optimized and tested to produce efficient and reproducible separations under UPLC® HILIC conditions. ACQUITY UPLC BEH HILIC columns overcome a major weakness of HILIC stationary phases: chemical stability. The unbonded silica stationary phases often used in HILIC are prone to dissolution at neutral pH. The rugged BEH particle’s wide usable pH range overcomes this pH limitation and results in long column lifetimes. ACQUITY UPLC™ BEH HILIC columns offer advantages such as retention and separation of very polar basic compounds, alternate selectivity as compared to reversed-phase chromatography, increased ESI-MS sensitivity, easier sample preparation, high efficiency and long column lifetimes.
