AmpliSeq for Illumina
Fast workflow and robust performance from a few to hundreds of genes, with as little as 1 ng input
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Easy to use and able to automate.
NGS
Ampliseq for Illumina is an easy NGS library preparation product to use. In particular, the hotspot cancer panel is very useful and covers most of the genes where interpretation is available.
Review Date: 28 Jan 2020 | Illumina
AmpliSeq for Illumina is a suite of AmpliSeq chemistry products that are compatible with Illumina next-generation sequencing platforms. The AmpliSeq for Illumina solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds in a single run.
Combined with Illumina next-generation sequencing (NGS) technology, AmpliSeq for Illumina sequencing offers high-confidence data to researchers in a wide variety of application areas, including cancer and genetic diseases. AmpliSeq for Illumina works with DNA and RNA samples and requires as little as 1 ng of input.
AmpliSeq panels can accommodate high-quality samples such as blood, cell culture, or fresh frozen tissue and also challenging samples such as formalin-fixed paraffin-embedded (FFPE) tissue. Select from predesigned panels or customize your content for a variety of genomes and unmatched flexibility.
AmpliSeq for Illumina products are only sold by Illumina and the complete workflow, from design to analysis, is supported by Illumina.
Expand Your Research Capabilities
Sequence with innovative NGS platforms that deliver exceptional data quality and accuracy, at any scale, with low hands-on time:
- Target variants for many applications with ready-to-use or custom panels
- Use a multiplexed, PCR-based workflow
- Transform data into insight with a variety of data analysis solutions
Sequence a Broad Range of Samples
AmpliSeq for Illumina is compatible with samples where available input quantity and quality are not limiting, such as blood, cell culture, or fresh-frozen tissues as well as challenging sample types, such as formalin-fixed paraffin-embedded (FFPE) tissue. The input requirement for DNA and RNA ranges from 1 ng to 100 ng, depending on the application needs. For most applications, we recommend 10 ng input per pool.
Prepare Libraries Quickly and Easily
Library preparation with AmpliSeq for Illumina is both fast and simple. Prepare an on-target, high-uniformity amplified library in as little as 5 hours with just 1.5 hours of hands-on time.
Analyze Hundreds of Genes at Once
AmpliSeq chemistry allows researchers to analyze hundreds of genes simultaneously with ultra-high multiplexed PCR with 12 to more than 24,000 amplicons in a single panel. Achieve complete coverage of large targets using multiple primer pools to create overlapping amplicons.
