Anti-Human IgG Fc (HFC) Probes
Binds human IgGs and human Fc-fusion proteins. Recommended for rapid screening for quantitation, off-rate ranking for antibodies, kinetic screening and epitope binning.
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Gator™ Human Fc (HFC) Probes are useful for measuring the concentration and kinetics of antibodies and proteins recognized by antihuman Fc. HFC probes utilize a high affinity anti-human Fc antibody and can capture the Fc region of human IgG1, IgG2, IgG3 and IgG4. Applications include quantitation of crude or purified samples, kinetics analysis of antibodyantigen to determine (kon , koff , KD ), off-rate screening, and isotyping of crude hybridoma cell lysate. These probes can be regenerated and reused for multiple experiments.