Anti-Mouse IgG Fc (MFC) Probes
Binds mouse IgGs and mouse Fc-fusion proteins. Recommended for rapid screening for quantitation, off-rate ranking for antibodies, kinetic screening and epitope binning.
Anti-Mouse IgG Fc (MFC) Probes
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Gator™ Mouse Fc (MFC) Probes are useful for measuring the concentrations and kinetics of antibodies and mouse-Fc fusion proteins. MFC probes have a high-affinity anti-mouse Fc antibody immobilized on the surface. The Fc region of mouse IgG1, IgG2a, IgG2b can be captured on this surface with a high affinity stable interaction. (Mouse IgG3 should be evaluated on a case-by-case basis). Applications include quantitation of mouse and rat IgGs, kinetics analysis of antibody-antigen to determine (kon , koff , KD ), off-rate screening, and isotyping of crude hybridoma cell lysates. These probes can be regenerated and reused in multiple experiments.
