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Anti-Phospholipid Screen IgG/IgM ELISA
High Quality Assays with Reproducible and Reliable Results
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An enzyme immunoassay test system to screen for the presence of IgG and IgM class autoantibodies against cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and beta-2-glycoprotein I in human serum or plasma.
The text system contains a mixture of highly purified cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and human beta-2-Glycoprotein I is bound to microwells. The determination is based on an indirect enzyme linked immune reaction with the following steps:
- Specific antibodies in the patient sample bind to the antigen coated on the surface of the reaction wells.
- After incubation, a washing step removes unbound and unspecifically bound serum or plasma components.
- Subsequently added enzyme conjugate binds to the immobilized antibody-antigen-complexes.
- After incubation, a second washing step removes unbound enzyme conjugate.
- After addition of substrate solution the bound enzyme conjugate hydrolyses the substrate forming a blue colored product.
- Addition of an acid stops the reaction generating a yellow end-product. The intensity of the yellow color correlates with the concentration of the antibody-antigen-complex and can be measured photometrically at 450 nm.
