AO/PI, Acridine Orange Stain, Propidium Iodide Stain

Name: AO/PI, Acridine Orange Stain, Propidium Iodide Stain
SKU: F23001 Acridine Orange/Propidium Iodide Stain (2 x 0.5 mL) / F23011 Acridine Orange/Propidium Iodide Stain, Sterile-filtered (2 x 0.5 mL) / F23002 Acridine Orange Stain (500 tests), 2 x 0.5 mL / F23003 Propidium Iodide Stain (2 x 0.5 mL)
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Logos BiosystemsF23001 Acridine Orange/Propidium Iodide Stain (2 x 0.5 mL) / F23011 Acridine Orange/Propidium Iodide Stain, Sterile-filtered (2 x 0.5 mL) / F23002 Acridine Orange Stain (500 tests), 2 x 0.5 mL / F23003 Propidium Iodide Stain (2 x 0.5 mL)Worldwide

Used in fluorescence cell counting, Acridine Orange/Propidium Iodide Stain (AO/PI), Acridine Orange Stain (AO), and Propidium Iodide Stain (PI) are used to assess cell viability. Acridine Orange/Propidium Iodide Stain causes viable nucleated cells to fluoresce green and nonviable nucleated cells to fluoresce red.

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Acridine Orange/Propidium Iodide Stain (AO/PI)

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Description

The use of Acridine Orange/Propidium Iodide Stain (AO/PI) has grown traction in recent years as a ‘rapid, highly linear, functionally correlated assay’ (Mascotti, 2000) when compared to Trypan Blue stain.

How it works: Acridine orange (AO) permeates viable cells and binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red.

Propidium iodide (PI) binds to nucleic acids. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes. Once bound to nucleic acids, its fluorescence increases 20 to 30-fold and causes the cell to fluoresce red.

Thanks to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results. Acridine Orange/Propidium Iodide Stain (AO/PI) is a pre-mixed formula containing both Acridine Orange (AO) and Propidium Iodide Stain (AO/PI).

AO Specifications: Molecular Formula: C17H20CIN3, Molecular Weight: 301.82 g/mol, Appearance: Clear liquid, Cell permeability: Membrane permeant, Excitation/emission: 500/526 nm (when bound to DNA) & 460/650nm (when bound to ssDNA or RNA).

PI Specifications: Molecular Formula: C27H34I2N4 Molecular Weight: 668.39 g/mol, Appearance: Clear liquid, Cell permeability: Membrane impermeant, Excitation/emission: 493/636 nm (in aqueous solution) & 533/617 nm (when bound to nucleic solutions).

AO/PI Specifications: Appearance: Orange-red liquid, Cell permeability: Membrane permeable (acridine orange) & Membrane impermeant (propidium iodide), Excitation/emission: 500/526 nm (acridine orange) & 533/617nm (propidium iodide).

Compatible with the following instruments: LUNA-FL™ Automated Cell Counter, LUNA-STEM™ Automated Cell Counter, and the LUNA-FX7™ Automated Cell Counter.