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Apyrase from potatoes

Merck KGaA, Darmstadt, GermanyA6535Available: Worldwide

ATPase ≥200 units/mg protein, lyophilized powder

Merck KGaA, Darmstadt, Germany

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Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

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