Atlantis T3 HPLC columns
The Atlantis® T3 HPLC columns set the standard for retention and separation of polar compounds via reversed-phase HPLC. Atlantis® T3 columns are a universal, silica-based, reversed-phase C18 line of HPLC columns that not only retain and separate small, water-soluble polar organic compounds, but also provide superior performance across a wider pH range. Atlantis® columns are the culmination of over 30 years of bonded phase rese…
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An alternative for polar compounds analysis.
Environmental analysis
A reversed-phase analytical column that allows the use of 100% aqueous mobile phase. An interesting and useful alternative to HILIC for the chromatographic separation of highly polar compounds.
Review Date: 2 Feb 2021 | Waters
Always recommend and as first choice for method development.
Analyze drug concentration in plasma
Good separation, robust and long lifetime.
Review Date: 18 Nov 2020 | Waters
Great product.
Reagents
Very satisfied with these columns.
Review Date: 30 Oct 2020 | Waters
Good separation and repeatable results.
Analysis of cyanotoxins in environmental samples
The Atlantis T3 column provided effective separation for several compounds with different structures and physicochemical properties. It can be used with a large percentage of water in mobile phase (i.e 95%) which is rare for a C18 column but it could be considered a little bit expensive.
Review Date: 26 Oct 2020 | Waters
Great results.
Analyze metabolites
By using this column we can estimate 7 compounds in single injection.
Review Date: 26 Jul 2018 | Waters
Overall it's a good product
pharmaceuticals
Gives reproducible and precise results, easy to operate. Good for method development and validation.
Review Date: 27 Jan 2017 | Waters
Great results
Analyze metabolites in pepper samples
Good separation in pepper samples. More efficiency
Review Date: 30 Nov 2015 | Waters
Reliable and requires little maintenance.
Residue and metabolism
The column has great performance it is reliable and requires little maintenance I recommended as a great tool
Review Date: 9 Nov 2015 | Waters
The Atlantis® T3 HPLC columns set the standard for retention and separation of polar compounds via reversed-phase HPLC. Atlantis® T3 columns are a universal, silica-based, reversed-phase C18 line of HPLC columns that not only retain and separate small, water-soluble polar organic compounds, but also provide superior performance across a wider pH range. Atlantis® columns are the culmination of over 30 years of bonded phase research and are a vast improvement upon the highly successful Atlantis® dC18 line of columns. For reversed-phase applications, Atlantis® T3 columns should be considered the first choice when developing a separation of polar (and non-polar) compounds. The advantages of T3 bonding technology are extended to UPLC® in the ACQUITY® UPLC® HSS T3 columns.
T3 Bonding
The superior performance of Atlantis® T3 HPLC columns and the ACQUITY® UPLC® HSS T3 columns is a result of Waters’ , advanced T3 bonding process. T3 bonding utilizes a trifunctional C18 alkyl phase bonded at a ligand density that promotes polar compound retention and aqueous mobile phase compatibility. The proprietary T3 endcapping process is much more effective than traditional trimethyl silane (TMS) endcapping.
The unique combination of bonding and endcapping provides superior polar compound retention and aqueous compatibility while also enhancing column performance, lifetime, peak shape and stability.
A Universal Reversed-Phase Polar Compound Retention Column
Large organizations are looking to narrow or limit the number of column choices available to their separations scientists. This worldwide LC column “harmonization” can provide more predictable, controlled consumable costs, simplified method transfer between laboratory locations around the world, and pre-screened LC columns for various performance parameters.
With good retention of, and selectivity for, neutral, hydrophilic compounds as well as polar analytes and superior performance over a wider pH range, Atlantis® T3 columns merit serious consideration as a more universal alternative to traditional C18 reversed-phase LC columns.
