B-HCG ELISA
High Quality Assays with Reproducible and Reliable Results
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Great and easy to use
Analysis in human plasma
Very easy and good value for the money. We can do many samples at once since the assay is very quick (compared to standard ELISAs) or can do only a few samples if needed. Results are reliable (from plate to plate)
Review Date: 12 Nov 2018 | DRG International Inc.
I use this product to measure hCG secretion by human trophoblast cells
The kit is easy for use and the results are robust. Also the support team was helpful in answering my questions.
Review Date: 30 Jan 2015 | DRG International Inc.
An enzyme immunoassay for the quantitative measurement of total human chorionic gonadotropin (hCG and b-hCG) in serum. Chorionic Gonadotropin (hCG) is a glycoprotein hormone which is normally produced by the placenta during pregnancy. After conception, the hCG concentration increases rapidly to reach a peak near the end of the first trimester. High concentrations are observed throughout pregnancy. After delivery, hCG levels fall rapidly and become undetectable after a few days. Structurally intact hCG molecules are composed of an alpha and a beta subunit. The alpha subunit is nearly identical to the alpha subunits of other glycoprotein hormones, such as Thyroid Stimulating Hormone (TSH), Luteinizing Hormone (LH), and Follicle Stimulating Hormone (FSH): The differences in the beta subunit of the respective hormones account for their biological specificity and immunochemical distinctiveness. Monoclonal antibodies recognizing unique sites on the beta chain of the ß-hCG/hCG molecule are essential for differentiation between hCG and LH, FSH and TSH. Specific assays for beta-hCG permit the early detection of pregnancy. In addition to the elevated hCG levels during pregnancy, high concentrations of ßhCG/hCG may be associated with neoplasms of trophoblastic and nontrophoblastic origin such as hydatiform mole, chorionepithelioma, embryonal cell carcinoma,seminoma and many others.The DRG Beta-HCG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody [mouse] directed towards a unique antigenic site on a Beta-HCG molecule. An aliquot of sample containing endogenous Beta-HCG and/or HCG is incubated in the coated well with enzyme conjugate, which is an anti- Beta-HCG antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount ofbound peroxidase is proportional to the concentration of Beta-HCG/HCG in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of Beta-HCG/HCG in the sample.
