Bio-Rad Bradford Protein Assays
based on the dye-binding properties of Coomassie Blue G-250
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Great results.Good for the money.
Measurement of total protein compared to a standrard portein
The Good: Simple to utilize, quick and hearty one-venture assurance of protein focuses, with great similarity to a wide range of reagents. The Bad: Since protein focus relies upon presence of arginines and hydrophobic amino corrosive buildups, the protein fixation fluctuates in various proteins relying upon the presence of these amino acids. More modest scope of protein recognition than with most tests for example Bio-Rad DC Assay. The Bottom Line: Bio-Rad's Quick Start™ pack gives the norms and location arrangements important to quickly and dependably measure protein focuses at various volumes and over a wide scope of protein fixations. The measure is viable with most arrangements utilized for protein segregations and purgings, aside from cleansers.
Review Date: 4 Apr 2022 | Bio-Rad
Highly recommended if you are opting to work on proteins
Determine the solubility of proteins
Bio-Rad Bradford protein assay kit is easy to use mainly for determining the solubility of proteins. It's analysis protocol is made much simpler as the kit provides all the steps and the standards as well at a great price. Highly recommended.
Review Date: 6 Dec 2021 | Bio-Rad
The Bradford assay is based on the use of the dye Coomassie Brilliant Blue G-250, which is frequently abbreviated as Coomassie G-250 or Coomassie Blue. This is one of two Coomassie dyes that are often confused. Coomassie R-250 is used to stain protein gels but is not used in protein assays. In addition to being used in the Bradford assay, Coomassie G-250 can also be used to stain protein gels, although it is less sensitive than Coomassie R-250.
Under acidic conditions, Coomassie G-250 is cationic, mainly doubly protonated, and is red, whereas in neutral conditions the dye is green, and the anionic form is blue. The Bradford reagent is an acidified solution of Coomassie G-250; the dye is thus primarily protonated and red.
The basis for the Bradford assay is that in order for the Coomassie dye to bind stably to protein, it needs to be doubly protonated. When the dye comes in contact with protein, the first electron is donated to charged groups on the protein. This disrupts the structure of the protein, resulting in exposure of hydrophobic pockets. The dye binds to these pockets, with the sulfonic acid groups binding to positive amines. In addition, there is attraction due to Van der Waals forces. The stably bound Coomassie G-250 is the blue, unprotonated form.
