Biocrates® Vitamin D Kit
The Biocrates® Vitamin D Kit provides ready-to-use components for a LC-MS/MS-based assay and enables the quantitative measurement of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3. The inability to differentiate between 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 is the main drawback of immune-based techniques. The separate measurements are useful for both, clinical and research purposes. Moreover, the cross reactivity with…
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The Biocrates® Vitamin D Kit provides ready-to-use components for a LC-MS/MS-based assay and enables the quantitative measurement of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3.
The inability to differentiate between 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 is the main drawback of immune-based techniques. The separate measurements are useful for both, clinical and research purposes. Moreover, the cross reactivity with other non targeted vitamin D metabolites can only be accounted statistically. It was found that an immunoassay overestimates 25-hydroxyvitamin D3 and underestimates 25-hydroxyvitamin D2 concentrations. For this reasons, LC-MS/MS based methods for the quantitation of total 25-hydroxyvitamin D replaced IAs in many laboratories and became the “golden standard”.
Features:
- Ready to use LC-MS/MS Kit for all major mass spectrometric platforms Biocrates-VitaminD-Kit-1
- Certified reference materials (traceable to the new NIST SRM 972a standard, DGKL ring trial)
- Fast and effective 3 – step sample preparation
- Removal of proteins and phospholipids from sample
- Three simultaneous results (25-hydroxy-vitamin D total, 25-hydroxy-vitamin D2, 25-hydroxy-vitamin D3)
