BIOTAQ™ DNA Polymerase
BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent. BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leav…
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It’s a great product to obtain good results.
PCR, Cloning and Expression
Easy to use, faster than the conventional Taq DNA polymerase, sensitive and reliable. Just loaded into the well of agarose on post-PCR. When I am facing some technical issue, I asked the technician; they gave a quick response and helpful. The price is worth for your research.
Review Date: 3 Mar 2017 | Bioline UK
Standard PCR
One of the cheapest Taqs available. Very easy to use when you only require PCR product band size estimation. Not ideal for cloning as it has no proof reading capabilities.
Review Date: 21 Sept 2015 | Bioline UK
BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.
BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.
BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.
The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.
BIOTAQ™ DNA Polymerase is purified from Thermus aquaticus.
