BIRB-796

BIRB-796 is a highly potent inhibitor of p38 MAPK (Kd = 0.1 nM) that blocks TNFα release in LPS-stimulated THP-1 cells (IC₅₀ = 18 nM) (Pargellis et al.). At 10 μM, BIRB-796 can also inhibit JNK2α2 in vitro, but at lower concentrations BIRB-796 inhibits p38 MAPK without affecting phosphorylation of JNK substrates (Bain et al.; Kuma et al.).

MAINTENANCE AND SELF-RENEWAL

• Rescues the self-renewal ability of muscle satellite cells from aged mice (Bernet et al.).
• Increases the regenerative capacity of functional aged skeletal muscle stem cells grown in hydrogel (Cosgrove et al.).
• Blocks GADD45G-induced differentiation of long-term repopulating hematopoietic stem cells (Thalheimer et al.).
• Enhances stem cell activity of cultured umbilical cord blood-derived hematopoietic cells cultured in serum-free medium supplemented with SCF, TPO, and FLT3L (Baudet et al.).
• Blocks the osteogenic differentiation effect of myocilin on human mesenchymal stem cells (Kwon et al.).