Ca++Pure-HA Chromatography Media
Ca++Pure-HA is a hydroxyapatite resin used in the chromatographic separation of biomolecules.
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I recommend this resin and Tosoh in general to everyone.
Protein purification polishing
I have been using Ca++Pure-HA for several years in the purification of proteins and antibodies. It became one of my favorite resins since it demonstrates high level of impurity removal 2-3 logs even when anything else fails. Ca++Pure-HA is much more stable than other ceramic resins available and it is very easy to scale up the process. The post marketing support is nothing less than amazing.
Review Date: 8 Feb 2020 | Tosoh Bioscience
Ca++Pure-HA is a hydroxyapatite resin used in the chromatographic separation of biomolecules.
Ca++Pure-HA is specifically developed for the purification of monoclonal and polyclonal antibodies, the separation of antibody isoforms, isozymes, the purification of antibody fragments, and the isolation of single-stranded from double-stranded DNA. Ca++Pure-HA resin has been sintered at high temperatures for increased mechanical and chemical stability, allowing it to withstand the rigors of industrial-scale applications.
Ca++Pure-HA has a demonstrated dynamic binding capacity (DBC), at 10% breakthrough, of greater than 20 g/L for lysozyme at residence times as low as one minute and greater than 30 g/L for human IgG at residence times as low as 2 minutes.
Ca++Pure-HA provides exceptional separation properties and unequalled selectivity and resolution for multiple classes of biomolecules. The highly selective and robust nature of Ca++Pure-HA offers the flexibility to use this resin at any stage in a process from capture to final polishing.
